Fig. 1
From: Detection and tracking of overlapping cell nuclei for large scale mitosis analyses
Large series of cell cluster acquisitions using Fibronectin micro-patterns. a shows an image displaying all micro pattern positions of a given field of view. This image is captured once at the beginning of the sequence to locate cell patterns. b shows an acquisition of one time frame of the H2B-mCherry signal for the same field of view. This image contains the cell clusters. c shows one of the pattern position (corresponding to the green square on the top right of the field of view in image (a) and (d) shows the corresponding cell cluster located on it. A movie is automatically extracted from each pattern positions containing cells. The thousands of movies extracted this way from multiple fields of view are then analyzed using the proposed method. Scalebars are 80 μ m for (a) and (b) and 20 μ m for (c) and (d)