Samples | Expected ER | SRER | SRER Bias | EER_CS | EER_CS Bias | EER_RS | EER_RS Bias |
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100217 | 0.0323 | 0.0250 | 0.0073 | 0.0315 | 0.0008 | 0.0315 | 0.0008 |
100514 | 0.0152 | 0.0143 | 0.0009 | 0.0152 | 0.0000 | 0.0152 | 0.0000 |
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aBased on 1000 replicates. The frequency-based simulation approach was applied. For each replicate, we considered the top 1000 reads with the highest frequencies as the error-free reads and generated 1000 pairs of error-free read counts and shadow counts
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Expected ER expected error rate in simulation studies
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SRER error rate estimated using shadow regression
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SRER Bias the absolute value of the difference between SRER and Expected ER
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EER_CS empirical error rate estimated using cubic smoothing spline
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EER_CS Bias the absolute value of the difference between EER_CS and Expected ER
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EER_RS empirical error rate estimated using robust smoothing spline
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EER_RS Bias the absolute value of the difference between EER_RS and Expected ER