Fig. 5
Analysis of the motion modes within a real trajectory of a Moloney Murine Leukemia virus (MLV) particle in HEK cells. The upper panel shows the trajectory of a MLV particle containing YFP-tagged Gag proteins recorded in infected 293 HEK cells, tracked using TIRF microscopy and recorded at a 100 ms integration time. The confinement area is zoomed in the red circle. The lower panel is the plot of the probability of detection of motion mode (BPNN output values) as a function of the duration time of the trajectory. A zone of confinement is detected during 6.1 s (bold red trace) with a diffusion coefficient of 0.01 μm2/s and a diameter of 170 nm. Directed motion is indicated during 4 s with a diffusion coefficient of 0.012 μm2/s and a velocity of 0.38 μm/s. Scale bar, 500 nm