Fig. 1

icSHAPE score profiles for the iron response element (IRE) hairpins of murine Ftl1 (a) and Fth1 (c) visualized using Structure Surfer’s standardized data output. The Ftl1 IRE is located at position 76 to 110 in the transcript and, in the genome, is located on chromosome seven from position 45459777 to 45459811 on the non-reference strand. Fth1’s IRE is located at position 83 to 117 in transcript variant 1. In the genome its coordinates are from 9982728 to 9982762 on the reference strand of chromosome 19. In vitro reactivity scores from the database are superimposed on in silico predicted structures for Ftl1 (b) and Fth1 (d) using SAVoR [4]. Red indicates positions with higher reactivity, showing evidence of low secondary structure. Positions colored in yellow have lower reactivity and are more likely paired