flowVS: channel-specific variance stabilization in flow cytometry

BMC Bioinformatics

Table 1 The ratio of between-cluster to within-cluster variations (a measure of effect size of metacluster homogeneity defined in Eq. 5) after four transformations

Cell populations	dataset	flowTrans	logicle	FCSTrans	flowVS
CD4⁺ metacluster	HD	2.8	13.70	13.42	.87
	ITN	1.3	1.55	1.54	.61
erronous CD4^-/CD4⁺ metacluster	HD	24.40	21.63	12.64	36.77
	ITN	11.68	11.30	10.38	26.47

CD4⁺ cell populations are used in top two rows, and a mixture of CD4⁺ and CD4^- cell populations are used in bottom two rows. Small and large values of the ratio denote homogeneous and heterogeneous collections of clusters, respectively. In this example, flowVS transformation results in the highest homogeneity when only CD4⁺ clusters are considered, and highest heterogeneity when CD4⁺ and CD4^- clusters are mixed together

ISSN: 1471-2105