Fig. 14
From: A model of the spatial tumour heterogeneity in colorectal adenocarcinoma tissue
Examples of segmentation results using ImageJ. Figures (a) and (b) show original data for a cancerous image and results from nuclear identification using the ITCN plugin followed by watershed segmentation to obtain nuclear boundaries. Red dots mark centres of detected regions. Figures (c) and (d) show original data for a healthy image and results from segmentation using thresholding and watershed segmentation. Figures (b) and (d) show the borders of the segmented regions with a red dot identifying the centres of the proposed segmented cells