Table 2 Cell counting results for CellProfiler (CP) and ImageJ(IJ) with ITCN (Image-based tool for counting nuclei) Plugin and with watershed segmentation. Counting based on non-overlapping nuclei or cytoplasm regions with L max =0.4. Mean ± standard deviation are shown normalized by the ground truth. A value over 1 shows over-segmentation, whereas a value under 1 demonstrates under-segmentation
From: A model of the spatial tumour heterogeneity in colorectal adenocarcinoma tissue
CellProfiler (CP) and | |||||
|---|---|---|---|---|---|
Image type | CP nuclei | CP cytoplasm | IJ nuclei ITCN | IJ nuclei | IJ cytoplasm |
All | 1.007 ± 0.014 | 0.919 ± 0.149 | 0.952 ± 0.036 | 1.094 ± 0.041 | 0.945 ± 0.283 |
Healthy | 1.014 ± 0.011 | 1.046 ± 0.084 | 0.976 ± 0.022 | 1.062 ± 0.023 | 1.139 ± 0.291 |
Cancer | 1.001 ± 0.015 | 0.792 ± 0.071 | 0.929 ± 0.031 | 1.125 ± 0.029 | 0.751 ± 0.021 |