Skip to main content
Fig. 2 | BMC Bioinformatics

Fig. 2

From: Analysis of amplicon-based NGS data from neurological disease gene panels: a new method for allele drop-out management

Fig. 2

Schematic representation of trimming algorithm, based on two separate steps of alignment and variant calling. The first step is characterized by primer sequence trimming, the second step by the removal of reads generated by primer pairs that pair in a mutated region, with the mutation identified during the first step of variant calling. Variants obtained in the two different steps are merged, annotated, and provided as output from this pipeline

Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us