Skip to main content

Advertisement

Table 3 Post-transcriptional modifications in mt-tRNAs

From: A comprehensive collection of annotations to interpret sequence variation in human mitochondrial transfer RNAs

Position Modified base name Modomics symbol Human mt-tRNAs Bovine mt-tRNAs Model organisms tRNAs
9 1-methyladenosine Asp, Leu(CUN), Lys, Pro Ala, Glu, Phe, Gly, His, Asn, Arg, Thr, Val, Trp  
9 1-methylguanosine K Ile, Leu(UUR) Cys, Gln, Tyr  
10 N2-methylguanosine L Leu(UUR), Leu(CUN), Lys, Asp   Ala, Phe, Gly, His, Asn, Val, Trp, Tyr
20 dihydrouridine D Leu(UUR)   
26 N2-methylguanosine L    Ala, Glu
26 N2,N2-dimethylguanosine R Ile   
27 pseudouridine P Asp, Ile, Leu(UUR), Leu(CUN), Lys, Met, Pro Cys, His  
28 pseudouridine P Ile, Lys, Leu(CUN), Pro Cys, Glu, Asn, Ser(UCN), Tyr  
29 pseudouridine P    Ser(UCN)
31 pseudouridine P Leu(CUN)   
32 3-methylcytidine    Ser(UCN), Thr
32 pseudouridine P Pro   Cys
34 5-taurinomethyluridine Ê Leu(UUR)   Glu, Gln, Trp
34 queuosine Q Asp   His, Asn, Tyr
34 5-taurinomethyl-2-thiouridine Ǝ Lys Glu, Gln  
34 5-formylcytidine > Met   
37 N6-threonylcarbamoyladenosine 6 Ile, Lys, Ser(AGY)   Asn, Thr
37 N6-isopentenyladenosine +   Cys, Phe, Ser(UCN), Trp, Tyr  
37 2-methylthio-N6-isopentenyladenosine *   Phe, Ser(UCN), Trp, Tyr  
37 1-methylguanosine K Leu(CUN), Pro Gln  
38 pseudouridine P Pro   
39 pseudouridine P    Ala, Cys, Phe, Gly, His, Gln, Arg, Tyr
40 pseudouridine P    Glu, Gln
48 5-methylcytidine ? Leu(UUR)   
49 5-methylcytidine ?    Glu
50 pseudouridine P Met   
54 5-methyluridine T Leu(UUR), Pro   
55 pseudouridine P Leu(UUR), Pro   Glu, Gln, Ser(UCN), Tyr
58 1-methyladenosine Ser(AGY), Leu(UUR) Cys, Ser(UCN) Glu
72 5-methylcytidine ?    Thr
  1. All the post-transcriptional modifications confirmed or predicted in human mt-tRNAs are listed. The full name of modifications, Modomics symbols and positions affected are shown for each tRNA species. Modifications reported include those confirmed by crystallographic data in eight human mt-tRNAs, those predicted using bovine model, which has similar structure and sequence in mt-tRNAs, and those predicted based on model organisms, such as bacteria, yeast and nematode