TY - JOUR AU - Consiglio, Arianna AU - Mencar, Corrado AU - Grillo, Giorgio AU - Marzano, Flaviana AU - Caratozzolo, Mariano Francesco AU - Liuni, Sabino PY - 2016 DA - 2016/11/08 TI - A fuzzy method for RNA-Seq differential expression analysis in presence of multireads JO - BMC Bioinformatics SP - 345 VL - 17 IS - 12 AB - When the reads obtained from high-throughput RNA sequencing are mapped against a reference database, a significant proportion of them - known as multireads - can map to more than one reference sequence. These multireads originate from gene duplications, repetitive regions or overlapping genes. Removing the multireads from the mapping results, in RNA-Seq analyses, causes an underestimation of the read counts, while estimating the real read count can lead to false positives during the detection of differentially expressed sequences. SN - 1471-2105 UR - https://doi.org/10.1186/s12859-016-1195-2 DO - 10.1186/s12859-016-1195-2 ID - Consiglio2016 ER -