Fig. 2
The proportion of reference alleles at heterozygous sites before and after normalization. Each plot shows the combined results from five simulated datasets, with the known reference proportion (ground truth) on the x-axis and the reference proportion obtained from aligning sequencing data (estimated) on the y-axis. a Shows the results obtained from initial mapping under four different approaches, and (b) shows the results of the same approaches after normalization. The sum of squared errors (SSE) is calculated around the red line (x = y), whereas R2 is obtained from analysing the correlation between the two variables