Fig. 5

Soma localization results derived by the proposed method. a The manual localizations from the image stack with the total size of 150 × 150 × 150 voxels and with the voxel size of 2 × 2 × 2 μm³. b Shows the localization of the somas derived by the proposed approach. The detailed results can be found in the enlargement of the region (circles). White dots indicate manually located positions of somas; red dots are the positions located by our method. Arrows and triangles indicate the false positive positions and the missing positions respectively. c Robustness of Gaussian kernel width on the localization. The evaluation indexes, including localization precision, recall and F ₁-measure, are obtained using our method to analyze this dataset and are plotted against kernel width