Fig. 2

Level of depletion in ribodepleted vs. control metatranscriptomes. Identical pilot metatranscriptome samples were sequenced; protocol 1 included a ribodepletion step, while protocol 4 did not include this step. As is demonstrated, not all species were equally depleted, skewing the perceived abundances of different organisms within the metatranscriptome. Normalized data shows the combined average depletion percentage for each phylum of bacteria present within the sample