Skip to main content
Fig. 4 | BMC Bioinformatics

Fig. 4

From: RNAdualPF: software to compute the dual partition function with sample applications in molecular evolution theory

Fig. 4

Z-scores of mutational robustness (a) and of plasticity (b) are presented for C. elegans microRNA from Rfam 12.0. Robustness and plasticity were measured as explained in Fig. 3. For each wild type (WT) sequence, 2000 sequences were sampled from RNAdualPF both with and without control over GC-content (default sample), while 500 sequences were generated by RNAdualPF, both with and without control over GC-content, to exactly fold into the target structure (MFE str. sample). This was achieved by repeatedly sampling sequences in order to obtain a sequence, whose MFE structure was identical to the target structure. The number of sequences necessary to sample in order to obtain one sequence that folds into the target structure was variable, depending on the target structure and GC-content – in many cases, only 10 samples were necessary per selected sequence, in some cases 200 samples were necessary. and in one specific case 5000 samples were required. By control over GC-content, we mean that all sampled sequences have identical GC-content to the wild type sequence

Back to article page