Fig. 2

Plot showing conformational differences in renin ^C20R. Outermost layer indicates reported SNVs (Single Nucleotide Variants; gray, not validated; red, non-synonymous; green, synonymous) from dbSNP 138. a. Conservation scores represented as a histogram (blue, signal peptide; green, propeptide; red, domain). These values are generated as described in section 5 and are not related to MODICT score. b and c. Amino acid sequences with residues colored according to their property (positively charged, red triangle; negatively charged, blue triangle, non-polar, gray circle; polar, pink circle; aromatic ring, green hexagon). d. Iterative MODICT scores of individual residue pairs (algorithm, Eq.1) resulting from comparison with renin ^WT and renin ^R33W. Each blue histogram bin designates the contribution of a residue pair to the overall MODICT score (Higher bars mean more contribution as well as more the adverse effect of that residue pair on structural stability). These histogram bins are generated by iterative MODICT algorithm and are colored according to conservation. e. Important regions, SNVs and Indels (insertion-deletions) are marked with boxes. Red boxes represent SNVs whereas pink boxes represent Indels. Gray bordered boxes represent unvalidated changes. (S-S = disulphide bond)