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Table 1 Barcode count having similar pairs within one bas

From: Genome-wide functional analysis using the barcode sequence alignment and statistical analysis (Barcas) tool

Library

Static sequence length comparison

Dynamic sequence length comparison

GeCKOv2.Human.A

517 (0.81%)

538 (0.84%)

GeCKOv2.Human.B

437 (0.77%)

441 (0.78%)

GeCKOv2.Mouse.A

736 (1.12%)

755 (1.14%)

GeCKOv2.Mouse.B

850 (1.39%)

860 (1.41%)

.yusa

517 (0.59%)

3,944 (4.51%)

Cellecta.Human.M1

0 (0 %)

412 (1.5%)

Cellecta.Human.M2

0 (0 %)

398 (1.45%)

Cellecta.Human.M3

0 (0 %)

410 (1.49%)

TRC

790 (1.28%)

1,909 (3.10%)

Cerevisiae

0 (0 %)

0 (0 %)

Pombe

0 (0 %)

0 (0 %)

  1. Dynamic sequence length comparison and static sequence length comparison: if comparing sequences between ‘AGCT’ and ‘ACTA’, then the mapped sequence patterns are ‘AGCT-’ and ‘A-CTA’. Based on ‘AGCT’, the sequence has similar pairs within two bases in static sequence length comparison and one base in dynamic sequence length comparison. Dynamic sequence length comparison method doesn’t consider backward hyphen because the sequence already finished. Barcas uses dynamic sequence length comparison while mapping reads to library sequences