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Table 2 Designed barcode patterns of libraries

From: Genome-wide functional analysis using the barcode sequence alignment and statistical analysis (Barcas) tool

Library

Static sequence length comparison

Dynamic sequence length comparison

Count

intra

Intra & inter

inter

Count

intra

Intra & inter

inter

GeCKOv2.Human.A

517

27 (5.22%)

3 (0.58%)

487 (94.20%)

538

30 (5.58%)

3 (0.56%)

505 (93.87%)

GeCKOv2.Human.B

437

0 (0.0%)

0 (0.0%)

437 (100.0%)

441

0 (0.0%)

0 (0.0%)

441 (100.0%)

GeCKOv2.Mouse.A

736

8 (1.09%)

0 (0.0%)

728 (98.91%)

755

10 (1.32%)

0 (0.0%)

745 (98.68%)

GeCKOv2.Mouse.B

850

6 (0.71%)

0 (0.0%)

844 (99.29%)

860

6 (0.7%)

0 (0.0%)

854 (99.3%)

.yusa

517

2 (0.39%)

0 (0.0%)

515 (99.61%)

3,944

3,415 (86.59%)

48 (1.22%)

481 (12.20%)

Cellecta.Human.M1

0

   

412

0 (0.0%)

0 (0.0%)

412 (100.0%)

Cellecta.Human.M2

0

   

398

0 (0.0%)

0 (0.0%)

398 (100.0%)

Cellecta.Human.M3

0

   

410

0 (0.0%)

0 (0.0%)

410 (100.0%)

TRC

790

18 (2.28%)

0 (0.0%)

772 (97.72%)

1,909

1,045 (54.74%)

12 (0.63%)

852 (44.63%)

  1. (Intra: similar barcodes are in the same gene. Inter: similar barcodes have different gene. Most libraries have more inter barcodes than intra barcodes within one base distinction