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Fig. 2 | BMC Bioinformatics

Fig. 2

From: SIMBA: a web tool for managing bacterial genome assembly generated by Ion PGM sequencing technology

Fig. 2

MapSolver and SIMBA visualizations of the comparison between the new and old assemblies of Cp258. a MapSolver alignment visualization among the whole-genome optical mapping (enzyme kpnI; the central barcode in the color red), the old assembly (NC_017945; the barcode above in the color blue), and the new assembly (performed by SIMBA; the barcode below also in the color blue). Dark blue lines in the barcodes represent restriction sites. Lines connecting barcodes represent similarity regions. b The old assembly (above) is ~60 Kbp smaller than the restriction map (center), that have a length near to the new assembly (below). c SIMBA visualization compares the Cp258 old assembly (horizontal line red above) with the new assembly (horizontal line light blue below). Red lines that connect the line above and the line below represent syntenic regions. The visualization shows: d regions undetected in the old assembly; e mis-assemblies in the old assembly; and (f) the length difference between the genomes. The visualization showed by SIMBA agrees with the MapSolver results. In addition, it gives more detailed information about the genome differences

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