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Table 2 Assemblies using SIMBA

From: SIMBA: a web tool for managing bacterial genome assembly generated by Ion PGM sequencing technology

Genome

NGS

Software

Contigs

Scaffolding method

Genome length (bp)

Total scaffolds

Reference

Corynebacterium pseudotuberculosis 258 (Cp258)

Ion PGM

Mira3

41

Optical mapping (enzyme kpnI)

2,369,817

15

Data not published.

Mira4

56

Minia

675

Newbler

27

SPAdes

58

Corynebacterium pseudotuberculosis 1002 (Cp1002)

Ion PGM

Mira3

9

Optical mapping (enzyme kpnI)

2,335,107

5

[18]

Mira4

12

Minia

2,425

Newbler

10

SPAdes

15

Corynebacterium pseudotuberculosis 12C (Cp12C)

Ion PGM

Mira3

25

Reference (Corynebacterium pseudotuberculosis PAT10)

2,337,451

9

[22]

Mira4

62

Minia

-

Newbler

17

SPAdes

631

Corynebacterium pseudotuberculosis VD57 (CpVD57)

Ion PGM

Mira3

11

Reference (Corynebacterium pseudotuberculosis 31)

2,337,177

6

[23]

Mira4

15

Minia

1,146

Newbler

9

SPAdes

10

Corynebacterium ulcerans FRC11 (FRC11)

Ion PGM

Mira3

-

Reference (Corynebacterium ulcerans 0102)

2,442,826

6

[24]

Mira4

30

Minia

21a

Newbler

9

SPAdes

8

Corynebacterium ulcerans 210932 (210932)

Ion PGM

Mira3

12

Reference (Corynebacterium ulcerans 0102)

2,484,335

2

[25]

Mira4

-

Minia

343

Newbler

8

SPAdes

18

Lactococcus lactis subsp. lactis NCDO 2118 (NCDO2118)

Ion PGM

Mira3

661

Reference (Lactococcus lactis KF147)

2,554,693

18

[26]

Mira4

-

Minia

-

Newbler

43

SPAdes

-

  1. aMinia assembled only 8,097 bp of the genome
  2. -The assembly fails for an unknown reason