Fig. 1
From: PARRoT- a homology-based strategy to quantify and compare RNA-sequencing from non-model organisms
Analysis strategy for quantifying transcriptomes without a reference genome. All reads from different transcriptomes are pooled together for de novo assembly. Assembled contigs are used for searching homologs. Contigs which are matched to the same homolog are used to construct a virtual transcript for later use in quantification of expression. The sequencing reads are mapped to the virtual transcripts. Expression level for each virtual transcript are represented as mapped read count, estimated RPKM and estimated TPM based the mapping results