Fig. 3
From: PARRoT- a homology-based strategy to quantify and compare RNA-sequencing from non-model organisms
Expression level and number of virtual transcriptomes from Cnidaria. a For each contigs generated from pooled assembly, the expression level in RPKM is calculated for each virtual transcript. This plot shows that the expression level for most contigs from these two transcriptome datasets are similar to each other. b Number of virtual transcripts in each GO cellular component category in each transcriptome. After searching the most similar sequence of contigs in NCBI NR and Swiss-Prot databases, the GO annotations for the best hits are used to provide functional annotations for the assembled contigs. For all contigs hitting the same transcript in NR database, PARRoT quantify the expressions from them altogether because they are likely to come from the same transcript which is called as a virtual transcript in the plot. PARRoT calculates how many virtual transcripts were found for each GO category in each transcriptome