Fig. 1

Use case overview. Drug action studies using metabolomics involve measuring global metabolomics following pathogen exposure to a compound, usually in parallel with other investigational drugs. Statistical analysis of the resulting data sets enables one to reduce the data and identify drug-specific changes to pathogen metabolism (top). Drug-specific metabolic changes, in turn, can be entered into a Pathway Tools SmartTable (middle) and examined relative to the pathogen’s metabolism. Exporting SmartTable contents to the cellular overview diagram (bottom left) may reveal the pattern of drug action in global metabolism, whereas a pathway collage (bottom right) depicts a more focused subset of metabolism based on pathways containing drug-specific metabolites