Table 1 High-level categories (indicated by stars) and characters implemented in MicroPIE
Categories*/characters | Example source sentences |
|---|---|
G + C Content* | |
%G + C | DNA G + C content is 33.1–34.4 mol%. |
Cell Morphology* | |
Cell Shape | Cells are slender, cylindrical, sometimes crooked rods that are 0.35–0.5 μm wide and 2.5 μm long and occur singly or in pairs, or in longer chains. |
Cell Diameter | Cells are 0.3–0.5 μm in diameter. |
Cell Length | In glucose broth, the bacilli are longer, up to 4 μm long, and often occur in pairs. |
Cell Width | In addition, cells have an outer diameter of 1.5–3.0 μm and width of 0.5–0.9 μm. |
Cell Relationship & Aggregations | A few strains grow in chains of 3 to 6 elements in glucose broth. |
Gram Stain Type | The cells are Gram-negative, long, slender rods or sometimes filaments, motile by gliding. |
External features | Cells are frequently occurring in chains and producing appendages in older cultures. |
Internal features | No spores are observed. |
Motility | Cells are non-motile, extremely pleomorphic |
Pigment Compounds | Pigmented red due to carotenoids. |
Growth Condition* | |
NaCl Minimum | Growth requires at least 1.7 M NaCl, optimally 3.0–4.3 M NaCl. |
NaCl Optimum | |
NaCl Maximum | |
pH Minimum | Growth occurs at temperatures in the range 38–68 °C (optimum, 60 °C) and at pH 1.8–4.0 (optimum, pH 3.0). |
pH Optimum | |
pH Maximum | |
Temperature Minimum | |
Temperature Optimum | |
Temperature Maximum | |
Salinity Requirement for Growth | Requires sea salts for growth. |
Aerophilicity | Strictly anaerobic. Obligately anaerobic. |
Magnesium Requirement for Growth | Mg2+ and Ca2+ enhance growth. |
Vitamins and Cofactors Used For Growth | Vitamin K1 and haemin are required for growth. |
Antibiotic Physiology* | |
Antibiotic Sensitivity | Sensitive to (μg per disc) tetracycline (30), rifampicin (25) and vancomycin (30), but resistant to ampicillin (25), erythromycin (15), streptomycin (10) and gentamicin (10). |
Antibiotic Resistant | The type strain is resistant to erythromycin. |
Colony Morphology* | |
Colony Shape | On MA, colonies are convex, translucent, shiny and smooth. |
Colony Margin | Colonies are golden-yellow, circular and convex, with an entire edge on MA. |
Colony Texture | On MA, colonies are convex, translucent, shiny and smooth. |
Colony Color | Colonies are golden-yellow, circular and convex, with an entire edge and a butyrous consistency on MA. |
Metabolic End Products and Secondary Metabolites* | |
Fermentation Products | Acid is produced from glucose and fructose. |
Other Metabolic Products | Acetoin is produced. |
Microbe-Host Interaction* | |
Pathogenic | Pathogenic for humans, causing wound infections. |
Disease Caused | Pathogenic for honeybees in natural and experimental oral infections. |
Pathogen Target Organ | Nodosus is infected hooves of sheep and goats. |
Haemolytic/Haemadsorption Properties | No hemolysis occurs on rabbit blood agar. |
Metabolic Substrate* | |
Organic Compounds Used Or Hydrolyzed | Utilize carbohydrates for growth. |
Organic Compounds Not Used Or Not Hydrolyzed | Arabinose, mannose, N-acetylglucosamine, maltose are used as sole carbon and energy source but not mannitol, gluconate, caprate, adipate, citrate and malate. |
Inorganic Substances Used | Does not require yeast extract for growth, and can use inorganic nitrogen sources such as sodium nitrate and ammonium chloride. |
Inorganic Substances Not Used | Does not require yeast extract for growth, and can use inorganic nitrogen sources such as sodium nitrate and ammonium chloride. |
Fermentation Substrates Used | Ferments proteinaceous compounds. |
Fermentation Substrates Not Used | And no acid is produced from glycerol, erythritol, L-arabinose, D-ribose, or potassium 5-ketogluconate. |