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Table 5 Comparisons of assembly annotatability

From: Combining independent de novo assemblies optimizes the coding transcriptome for nonconventional model eukaryotic organisms

  

Illumina derived datasets

Simulated datasets

 

Assembler

L. stagnalis

S. cerevisiae

Caenorhabditis sp.

D. simulans

H. rhamnoides

N. benthamiana

D. melanogaster

C. elegans

Number of uniCDS

Concatenated

59,178

9,942

40,116

27,735

63,092

131,656

12,118

14,890

CLC

21,527

5,673

15,466

15,988

29,898

51,151

8,113

11,853

IDBA_tran

36,726

5,608

21,384

19,556

38,353

79,612

8,271

11,069

Trinity

44,545

9,339

34,356

23,509

46,571

88,428

11,351

12,838

Overall number of BLASTx hits

Concatenated

38,838

9,922

25,502

19,789

49,565

93,781

9,007

9,751

CLCa

14,034

5,666

9,983

11,221

23,523

36,587

5,777

7,740

(36%)

(57%)

(39%)

(57%)

(47%)

(39%)

(64%)

(79%)

IDBA_trana

23,634

5,598

13,996

14,107

30,491

56,665

6,218

7,489

(61%)

(56%)

(55%)

(71%)

(62%)

(60%)

(69%)

(77%)

Trinitya

30,134

9,320

21,730

16,765

36,232

63,079

8,342

8,559

(78%)

(94%)

(85%)

(85%)

(73%)

(67%)

(93%)

(88%)

Number of unique BLASTx hits

Concatenated

15,232

5,404

9,242

9,575

15,524

16,700

4,957

5,767

CLCa

10,958

5,094

7,492

8,376

12,405

12,902

4,664

5,529

(72%)

94%)

(81%)

(87%)

(80%)

(77%)

(94%)

(96%)

IDBA_trana

12,893

5,223

8,069

8,868

13,840

14,900

4,655

5,302

(85%)

(97%)

(87%)

(93%)

(89%)

(89%)

(94%)

(92%)

Trinitya

14,124

5,243

9,051

9,174

14,314

14,855

4,817

5,340

(93%)

(97%)

(98%)

(96%)

(92%)

(89%)

(97%)

(93%)

  1. aEach value is also expressed as a percentage of the corresponding Concatenated dataset value (numbers in brackets)