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Fig. 5 | BMC Bioinformatics

Fig. 5

From: Structural prediction of the interaction of the tumor suppressor p27KIP1 with cyclin A/CDK2 identifies a novel catalytically relevant determinant

Fig. 5

Analysis of cyclin A/CDK2 kinase activity on p27KIP1 substrate peptides. Cyclin A/CDK2 Kinase purified from Sf9 cells was incubated with increasing concentrations of p27KIP1 wild type and the R194A mutant peptide as indicated. Phosphorylation of the peptides was monitored after immobilisation on SAM2 membrane by scintillation counting and analysis by GraphPad Prism software. Shown are mean values ± SD of 5 experiments performed in duplicates

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BMC Bioinformatics

ISSN: 1471-2105

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