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Table 1 Comparison of Multiplex Primer Design software to existing primer design software

From: MPD: multiplex primer design for next-generation targeted sequencing

  Software
Feature MPD BatchPrimer3 MPprimer PCRTiler Primer-BLAST PrimerPlex
Multiple overlapping amplicons for genomic regions Yes No No Yes No No
SNP-specific amplicons Yes No No No No Yes
Multiplex compatible primers Yes No No No No Yes
Input BED Fasta sequence Fasta sequence Fasta sequence Fasta sequence dbSNP id or Fasta sequence
License GNU GPL v3 GNU GPL v2 GNU GPL v3 GNU GPL v3 GNU GPL v2 Comercial