Table 1 Comparison of Multiplex Primer Design software to existing primer design software
From: MPD: multiplex primer design for next-generation targeted sequencing
| Â | Software | |||||
|---|---|---|---|---|---|---|
Feature | MPD | BatchPrimer3 | MPprimer | PCRTiler | Primer-BLAST | PrimerPlex |
Multiple overlapping amplicons for genomic regions | Yes | No | No | Yes | No | No |
SNP-specific amplicons | Yes | No | No | No | No | Yes |
Multiplex compatible primers | Yes | No | No | No | No | Yes |
Input | BED | Fasta sequence | Fasta sequence | Fasta sequence | Fasta sequence | dbSNP id or Fasta sequence |
License | GNU GPL v3 | GNU GPL v2 | GNU GPL v3 | GNU GPL v3 | GNU GPL v2 | Comercial |