Fig. 1

Monocyte isolation by flow cytometry and sequence read characteristics. a Gating strategy for isolation of monocyte subsets, and a representative demonstration of increasing purity of monocyte subsets upon successive rounds of flow cytometric sorting. b Average base quality across all bases within a sample. c Total reads per sample. d Representative example of total reads mapped to the human genome. Class, classical monocytes; NC, nonclassical monocytes