From: TipMT: Identification of PCR-based taxon-specific markers
Tool | Sequence input | Search for Target sequences | Specificity check method | Output | Multiplex check |
---|---|---|---|---|---|
BatchPrimer3 | multiple sequences (up to 500) | yes (SSR, SNP) | none | primers information | no |
jPCR | multiple sequences | yes (SSR only) | alignment | primers information | yes |
MPprimer | multiple sequences | no | thermodynamic (limited and pre-defined list of sequences) | primers information and virtual gel | yes |
TipMT | multiple sequences from multiple taxa | yes (SSR, orthologs, singletons) | alignment and thermodynamic | primers information and virtual gel | yes |