From: Mixture model normalization for non-targeted gas chromatography/mass spectrometry metabolomics data
 | RSD % of individual metabolites across samples: mean (min, max) | |
---|---|---|
QC | Analytical | |
Truth | 2.99 (1.77, 4.32) | 5.82 (2.65, 16.15) |
Not normalized | 10.03 (2.33, 18.81) | 10.88 (2.88, 19.04) |
Mean centering | 3.08 (1.09, 6.08) | 5.24 (1.81, 14.72) |
Median scaling | 3.08 (.97, 6.30) | 5.32 (1.84, 14.97) |
Quantile | 10.21 (1.02, 18.69) | 10.66 (2.29, 18.27) |
Quantile + ComBat | 3.94 (1.10, 11.70) | 5.65 (1.62, 19.01) |
EigenMS | 6.82 (1.75, 15.27) | 7.05 (1.76, 16.05) |
VSN | 9.99 (2.27, 17.51) | 10.84 (2.81, 19.27) |
Batch normalizer | 1.73 (.19, 3.09) | 6.26 (2.41, 16.71) |
mixnorm | 2.42 (.73, 4.59) | 5.81 (1.84, 19.62) |