Table 1 Tools and databases integrated and implemented in VacSol

BLAST+2.2.25-7

New command line sequence alignment application developed using the NCBI C++ toolkit.

[38]

Pftools2.3

Package of programs that support the search method of generalized profile formatting.

[39]

PSORTb3.0

Protein subcellular localization prediction tool.

[17]

HMMTOP 2.0

Transmembrane topology prediction tool.

[32]

DEG 10.0

Database of essential genes.

[28]

VFDB

Virulence factors database.

[31]

ABCPred

B-Cell epitope prediction tool.

[40]

Propred-I

Prediction of promiscuous major histocompatibility complex (MHC) Class-I binding sites.

[41]

Propred

Prediction of MHC Class-II binding regions in an antigen sequence.

[42]

UniProt-SwissProt

Manually annotated protein sequences database with information extracted from literature.

[23, 33]