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Fig. 1 | BMC Bioinformatics

Fig. 1

From: Exploring the transcriptome of non-model oleaginous microalga Dunaliella tertiolecta through high-throughput sequencing and high performance computing

Fig. 1

Pipeline of RNA-Seq data analysis workflow from short sequence raw data. The general pipeline includes workflow for transcriptome database construction, annotation, and differential gene expression analysis. Workflow in data center mainly consists of de novo assembly, mpiBLASTX. Dt_G and Dt_KR are in-house constructed samples. Dt_Shin and Dt_Yao_v10 are two published datasets. 10% of each datasets are randomly picked for mpiBLASTX test to get the best parameters for mpiBLASTX with NCBI database for the merged D. tertiolecta datasets. The mpiBLASTX output was further extracted and filtered for annotation using python scripts

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BMC Bioinformatics

ISSN: 1471-2105

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