Table 1 The bioinformatic tools adopted for the prediction of secretory proteins from Puccinia helianthi transcriptome

SignalP v4.1

N-terminal signal peptides

http://www.cbs.dtu.dk/services/SignalP/

TMHMM v2.0

Transmembrane domains

http://www.cbs.dtu.dk/services/TMHMM/

Big-PI predictor

GPI-anchor site

http://mendel.imp.ac.at/gpi/fungi_server.html

TargetP v1.1

Secretion pathway and position

http://www.cbs.dtu.dk/services/TargetP/

ProtComp v9.0

Localization sequences

http://linux1.softberry.com/

LipoP v1.0

Lipoprotein signal peptides

http://www.cbs.dtu.dk/services/LipoP/

TatP v1.0

Signal peptide with RR-motif

http://www.cbs.dtu.dk/services/TatP/

Clustal Omega

Proteins homology prediction

http://www.ebi.ac.uk/Tools/msa/clustalo/