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Table 2 Amino acids frequency and distribution in cleavage sites of signal peptide of secretory proteins

From: The prediction of a pathogenesis-related secretome of Puccinia helianthi through high-throughput transcriptome analysis

Kinds of aa 20 amino acid residues at the cleavage position from - 3 to + 3 of the signal peptides
−3 −2 −1 +1 +2 +3
No. Percentage (%) No. Percentage (%) No. Percentage (%) No. Percentage (%) No. Percentage (%) No. Percentage (%)
V 242 26.7 34 3.7 3 0.3 50 5.5 34 3.7 72 7.9
A 147 16.2 40 4.4 446 49.1 94 10.4 18 2.0 53 5.8
S 135 14.9 150 16.5 148 16.3 96 10.6 97 10.7 72 7.9
T 103 11.3 42 4.6 47 5.2 44 4.8 57 6.3 68 7.5
I 81 8.9 27 3.0 1 0.1 30 3.3 36 4.0 76 8.4
C 72 7.9 14 1.5 70 7.7 15 1.7 22 2.4 43 4.7
L 45 5.0 137 15.1 18 2.0 84 9.3 59 6.5 89 9.8
G 34 3.7 9 1.0 123 13.5 33 3.6 29 3.2 36 4.0
F 6 0.7 53 5.8 3 0.3 30 3.3 25 2.8 35 3.9
H 6 0.7 48 5.3 0 0.0 33 3.6 22 2.4 24 2.6
R 6 0.7 26 2.9 6 0.7 41 4.5 34 3.7 13 1.4
K 6 0.7 24 2.6 0 0.0 30 3.3 50 5.5 38 4.2
N 5 0.6 57 6.3 3 0.3 40 4.4 60 6.6 49 5.4
E 5 0.6 77 8.5 5 0.6 66 7.3 86 9.5 39 4.3
M 4 0.4 15 1.7 4 0.4 11 1.2 4 0.4 12 1.3
Y 4 0.4 31 3.4 0 0.0 26 2.9 18 2.0 16 1.8
Q 3 0.3 73 8.0 10 1.1 117 12.9 42 4.6 45 5.0
W 2 0.2 15 1.7 3 0.3 3 0.3 4 0.4 13 1.4
D 1 0.1 31 3.4 5 0.6 58 6.4 59 6.5 43 4.7
P 1 0.1 5 0.6 13 1.4 7 0.8 152 16.7 72 7.9