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Table 2 The comparison of different normalization methods

From: metaX: a flexible and comprehensive software for processing metabolomics data

Methods NO. of peaks NO. of peaks (CV ≤ 30%)a DEFb Mean (CV) CHD d Mean (CV) Health d Mean (CV) QC e
ComBat 1438 930 127 0.4261 0.3816 0.1636
none 1438 527 65 0.4865 0.4739 0.2114
QC_RSC 1438 1191 178 0.5108 0.4664 0.1098
SVR 1438 1293 170 0.4853 0.4583 0.1081
PQN 1438 793 125 0.4945 0.4681 0.1777
Quantiles 1438 740 118 0.4911 0.4646 0.1895
sum 1438 761 119 0.5044 0.4733 0.1979
VSN 1438 772 120 0.5014 0.4761 0.1912
  1. Note:
  2. aAfter normalization, the number of peaks with CV ≤ 30% in QC samples
  3. bDEF: differentially expressed features with q-value < = 0.05, fold change > = 1.5 or fold change < = 0.667 and VIP > = 1
  4. cMean (CV) CHD: The average CV of peaks in CHD disease group
  5. dMean (CV) Health: The average CV of peaks in health group
  6. eMean (CV) QC: The average CV of peaks in QC group