Fig. 4

Percent Peptide and Transcript Representation. a The Guide Picker tool uses percent peptide as an indicator of location within the master coding DNA sequence (MCDS) of a gene. A score lower than 50% means the guide RNA targets closer to the 5′ end of the MCDS, while a score greater than 50% means the guide RNA targets toward the 3′ end of the gene. b Transcript representation refers to the number of gene transcripts this guide RNA can target with the same gRNA sequence. c Plotting percent peptide and transcript representation together reveals consensus exons throughout the MCDS of a given gene. In this example, most consensus exons appear toward the 3′ end of the gene. Data shown for the human (GRCh38) FAM3B gene. Introns are not drawn to scale