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Fig. 6 | BMC Bioinformatics

Fig. 6

From: Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments

Fig. 6

Designing a Tiling Experiment with Guide Picker. a First, guides are plotted along the length of the MCDS using percent peptide on the x-axis. The y-axis is set to Doench 2016 Positionless. b Filtering guides to a strict score threshold (e.g. >55) offers a panel of guides that still target along the full length of the MCDS. c The right-hand plot can then be set and filtered according to other guide parameters, such as GC (30–70%) content and Hsu 2013 (>68) off-target scoring. d The guides in this range can then be highlighted to verify that this select group of guides still target along the full length of the MCDS in the left-hand plot

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BMC Bioinformatics

ISSN: 1471-2105

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