Fig. 1From: ImmunoNodes – graphical development of complex immunoinformatics workflowsHLA ligandomics workflow combining native KNIME, OpenMS, and ImmunoNodes nodes. The workflow extracts MS data from PRIDE (FTP Connection and Download node) and performs mass spectra identification with the peptide search engine X!Tandem (XTandemAdapter), annotates the results with details of the given target/decoy database (PeptideIndexer), calculates false discovery rates (FalseDiscoveryRate) and filters for 5% FDR (IDFilter) using OpenMS’ nodes. The identified peptides are annotated with their respective binding affinity predicted by NetMHC using the EpitopePrediction node. Finally, simple summary statistics and visualizations are generated with the use of native KNIME nodesBack to article page