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Table 1 Representatives of families matching HMMs of well-characterized lipolytic proteins

From: In silico approach to designing rational metagenomic libraries for functional studies

Family/Representative

Members with complete sequence

Associated HMM from PFAM

Well-characterized enzymes from UniProt with accession number

FUMEFAM011958/GOS54

383

Alpha/beta hydrolase fold PF07859

Acetyl esterase EcE Escherichia coli P23872

FUMEFAM010194/GOS55

376

GDSL-like Lipase/Acylhydrolase family PF13472

Arylesterase Streptomyces coelicolor Q9S2A5, Lipase Streptomyces rimosus Q93MW7

FUMEFAM018084/GOS88

22

Alpha/beta hydrolase fold PF00561

Pimeloyl-[acyl-carrier protein] methyl ester esterase P13001

FUMEFAM012527/GOS89

7

PB011927

Thermostable organic solvent tolerant lipase Bacillus sp. Q5U780 (EC 3.1.1.3)

  1. Reviewed amino acid sequences with the maximal annotation score of 5 and bacterial origin were downloaded from UniProt [27]. Based on matching HMMs from Pfam (Release 27.0) we determined the 4 protein families with the highest homology to the well-characterized proteins from UniProt