Fig. 1From: An integrated enhancement and reconstruction strategy for the quantitative extraction of actin stress fibers from fluorescence micrographsTIRFM images of F-actin and the analysis pipeline for stress fiber extraction a Analysis pipeline for image enhancement and segmentation of stress fibers. b TIRFM images of U2OS cells plated on Y- (1), crossbow- (2) and disc-shaped (3) micropatterns (red boxes) with red, green and blue arrows indicating regions of stress fiber branching. Scale bar, 5 μm. c Enlarged images of regions highlighted by red, green and blue arrows in (1) and (2) of (B). Scale bar, 2 μmBack to article page