Fig. 4

Assessment of filament reconstruction accuracy. a Synthetic ground truth image containing both curved and straight lines, mimicking stereotypical stress fiber arrangement. b Synthetic image with PSNR of 20 dB. c 9 Detected filaments shown in different colors from (B). d-i) Maps of False positives (D-F) and false negatives (G-I) for the accuracy of individual filaments detection (described in text) are computed as a function of image noise (PSNR) versus filter radius (D, G), search radius (E, H), and search angle (F, I), respectively. Pixel size for synthetic images, 108 nm