Fig. 6

Extraction and quantitative analysis of stress fibers network. a–c Overlay of aligned U2OS cells plated on Y- (A), crossbow- (B) and disc-shaped (C) micropatterns. Colorbar, intensity. d–f) Overlay of detected stress fibers from aligned U2OS cells plated on Y- (D), crossbow- (E) and disc-shaped (F) micropatterns. Filaments with the same colors are from the same cell. g, h Comparision of the length (G) and width (H) of ventral stress fibers in cells plated on Y- (A) and crossbow-shaped (B) micropatterns. ***: p < 2x10^-21, t-test. ****: p < 5x10^-8, t-test. I, J) Histograms of F-actin orientations in protrusive regions of cells plated on crossbow- (I) and disc-shaped (J) micropatterns. Green lines: 3rd order polynomial fit. k, l Normalized actin density as a function of distance levels in cells seeded on crossbow- (K) and disc-shaped (L) micropatterns. Light red and green bands depict their standard deviations. n = 10 cells for each micropattern