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Fig. 3 | BMC Bioinformatics

Fig. 3

From: quantGenius: implementation of a decision support system for qPCR-based gene quantification

Fig. 3

Screenshot of the individual gene calculations. The calculations are done in three steps. 1) QC parameters CqLOQ, CqExtC, slope range, and slope difference and calculation mode are defined by the user. 2) Standard curve is reviewed for possible outlier reactions. 3) Sample reactions are reviewed. The pipetting error (red circle) causes deviations from the predefined QC parameters (red arrows). All formulas used for the calculations can be viewed (blue arrow)

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