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Table 1 Comparison of selected features of quantGenius and other software tools

From: quantGenius: implementation of a decision support system for qPCR-based gene quantification

Analysis tool/Feature Quantification method Multiple reference genes Quality control factor
Replicates Extraction control LOQ LOD Sample efficiency gDNA
quantGenius Std.curve + + + + + + -
GenEx ΔΔCq-E/Std.curve + + - - + - +
qBase+ ΔΔCq-E + + + - - - -
REST ΔΔCq-E + + - - - - -
DAG Expression Std.curve + + - - - - -
  1. Quantification method, use of multiple reference genes for normalization and implementation of QC factors in quantification are compared. Std.curve – standard curve, ΔΔCq-E – efficiency corrected ΔΔCq method, replicates – replicate variability, extraction control – extraction efficiency, LOQ/LOD imputation – identification and imputation of copy numbers that are under LOQ or LOD, respectively, sample efficiency – individual sample efficiency estimate, gDNA – gDNA contamination correction