Fig. 1

Example output from ReTrOS-Smooth applied to protein data. Data is from a luciferase reporter for the CAB clock gene in wild-type Arabidopsis plants (ROBuST Project) [15]. Panel a) shows the raw input data (blue circles), the kernel-smoothed input data (green) and the median fitted protein output (black). Panel b) shows the back-calculated median reporter mRNA expression (red) with 2.5% and 97.5% percentiles in dashed lines. Panel c) shows the back-calculated median transcription activity (blue) with the same percentiles as (b). The model in Eqs. (5) and (6) are used here. Degradation rates (mean, std. dev.) of mRNA and protein are (2.3, 0.46) and (0.13, 0.010), respectively, set by choosing luc reporter in the parameter setting GUI (Fig. 5, upper panel). For other parameter values, see the middle panel of Fig. 5