Fig. 5

The absolute difference in TPM estimates from polyA+ and ribo-minus libraries was reduced when applying a ratio-based correction to the latter. The line y = 1 is shown in red. The data shown is for BMDMs prior to LPS stimulation, quantified using the full Oar v3.1 transcriptome (n = 10,667 genes). As data is shown on a logarithmic scale, values of 0 are excluded. To reduce noise, genes with TPM < 1 either before or after correction were excluded