Table 2 Comparison of alignment criteria of Ub-ISAP and VISA
Alignment criteria | Ub-ISAP | VISA |
|---|---|---|
Alignment score | 100 | >60 |
Percent identity | 100 | >92 |
IS distance from query start site (QSS) | 0 bp | 3 bp |
Acceptance for IS calling | Reads aligning at more than one position are rejected | Top 5 candidates highest alignment score) |