Fig. 3From: ACE: an efficient and sensitive tool to detect insecticide resistance-associated mutations in insect acetylcholinesterase from RNA-Seq dataThe principles of the ACE pipeline. Raw reads were mapped with insect ace gene sequences by using Bowtie 2. Then, the resistant fragment (11Â nt) and susceptible fragment (11Â nt) flanking the mutation sites were used to scan the reads mapped with the insect ace gene. The scanned reads were divided into two types: resistant reads and susceptible readsBack to article page