Fig. 14
From: CRISPulator: a discrete simulation tool for pooled genetic screens
Experimental data from FACS-based CRISPRi screens resembles simulated data shown in Fig. 6. Grey dots: non-targeting sgRNAs, dots on a red-white-blue color scale: targeting sgRNAs. Number of deep sequencing reads for each sgRNA in two populations separated based on a fluorescent reporter signal are shown. a Screen carried out with high representation at all stages. b Screen with low representation at the infection stage. c Screen with low representation at the selection stage