Fig. 4
From: CRISPulator: a discrete simulation tool for pooled genetic screens
An example sgRNA activity distribution for a simulated CRISPRi library. The 80–90% high quality guides is typical for second-generation CRISPRi [8] libraries. We define high quality sgRNAs as sgRNAs that have high activity and lead to a > 60% knockdown. Low quality sgRNAs are essentially indistinguishable from the negative controls and will lead to minimal effects on phenotype as they cause <20% knockdown of a given gene