Fig. 7

Sample results from a CRISPulator simulation of a CRISPRi FACS-based screen. Top row: Each point represents and individual sgRNA, plotting its read numbers in the simulated deep sequencing run for the “low reporter signal” bin and the “high reporter signal” bin. sgRNAs are color-coded to indicate whether they target a gene with a positive phenotype (knockdown increases reporter signal, blue), a gene with a negative phenotype (knockdown decreases reporter signal, red), a gene without phenotype (grey), or whether they are non-targeting control sgRNAs (black). Bottom row: Based on the observed sgRNA phenotypes, gene phenotypes are calculated (mean log₂ ratio of read frequencies in “high” over “low” bins), and a gene P value is calculated to express statistical significance of deviation from wild-type. These are visualized in volcano plots in which each dot represents a gene. Genes are color-coded to indicate the actual phenotype: positive, blue; negative, red; no phenotype, grey